Figure 3

Periostin expression of basal keratinocytes and the presence of periostin in the supernatant are increased in cutting-type wound healing and cultured salt-split models but not in tape-stripping models. Representative images of hematoxylin–eosin and periostin immunofluorescence staining (a) in tape-stripping models at 24 and 72 h post-treatment (n = 3), (b) in cutting-type ex vivo skin wound healing models at 0 and 24 h post wounding (n = 5, basal keratinocytes are highlighted by dotted lines, magnification in the hematoxylin–eosin stained wound bed: 2,5x, bar = 250 µm), and (c) in cultured salt-split basement membrane injury models at 24 and 72 h post-injury (n = 5). Dotted rectangles indicate all the enlarged regions, magnification: × 20. Bar = 50 µm. The graphs show mean ± SEM (n = 3) of the periostin presence in the supernatant of the (d) ex vivo wound healing at 0 and 24 h, and (e) cultured salt-split models at 0, 24 and 72 h using western blot analysis. Data were normalized to 0 h control samples, **P < 0.01, determined by one-tailed two-sample t-test; *P < 0.05, determined by one-way ANOVA followed by Tukey’s posthoc test. Band intensities of periostin were quantitated with Image Studio software (LI-COR Biosciences, Lincoln, NE). Representative blots are shown.