Figure 2
Interaction of the C-terminal domain of CK1δ with the isolated kinase domain. (a) Overlay of a [15N, 1H]-BEST-TROSY-HSQC of the 15N-labeled non-phosphorylated C-terminus (red) with a spectrum of the same sample after adding a 1:1 ratio of the unlabeled kinase domain (blue). (b) Overlay of [15N, 1H]-BEST-TROSY-HSQC spectra of a titration of the phosphorylated C-terminal domain (red) with increasing concentrations of the kinase domain. The signals of the phosphorylated residues are low field shifted relative to the unphosphorylated amino acids. The two inlets present regions of the spectrum showing interaction with the kinase domain.
