Figure 1 | Scientific Reports

Figure 1

From: Discovery of a cystathionine γ-lyase (CSE) selective inhibitor targeting active-site pyridoxal 5′-phosphate (PLP) via Schiff base formation

Figure 1

(a) Structure, fluorescence quantum yields and detection mechanism of HSip-1, an H2S-detecting fluorescence probe that utilizes the complexation of HS− with Cu2+. (b) Detection mechanism of the enzymatic activity of CSE for inhibitor screening with HSip-1. (c) The result of HTS for CSE inhibitors from a chemical library of 161,600 compounds. (d) Structure of hit compound 1, and its inhibitory activity towards rCSE (mean ± S.E.) determined by fluorescence assay with HSip-1. Error bars represent ± S.D. (n = 4). (e) Inhibitory activity of 1 toward the production of sulfane sulfur (S0)-containing cysteine persulfide by rCSE (mean ± S.E.). Sulfane sulfur was detected with the fluorescence probe SSP4 (Dojindo Laboratory). Error bars represent ± S.D. (n = 4).

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