Table 4 List of oligonucleotide sequences used in the PCR reactions that were designed independently.
From: Ceramide synthase CERS4 gene downregulation is associated with KRAS mutation in colorectal cancer
Gene | Sense (5′–3′) | Antisense (5′–3′) |
|---|---|---|
ACTB | ATGAAGATCAAGATCATTGCTCCTC | ACATCTGCTGGAAGGTGGACA |
CERS1 | CCATGACCCACCATCTGTCT | TTGGTGAACTCAAGCTGCAC |
CERS2 | TCTATATCACGCTGCCCCTG | CTTGCCACTGGTCAGGTAGA |
CERS3 | ACTGTTGCTGGAATTGCGTT | TGTTACAGGTCTGCGTCCAT |
CERS4 | TCTCTGGTGCTGCTGTTACA | AGATGAGGAAGAGAGCGTCG |
CERS5 | CATCTTCTTCGTGAGGCTGC | TGTCCTGATTCCTCCGATGG |
CERS6 | ACCTGAAGAACACGGAGGAG | CCAGCAATGCCTCGTATTCC |
ASAH1 | TCAGGACCAACGTACAGAGG | AAAAGGGCCAGGAAAGTTGC |
ASAH2 | TGCACAGGACAAGTAGCAGA | CCATGATGAAGGCACGACTG |
ACER1 | CCTATCAGAGCTCCGAGGTG | AGTGGCCCGAAGATGAAGAA |
ACER2 | TCTCTCTGATGACCCTGGGA | GAGGCAGCATCAAAGTAGGC |
ACER3 | ACTGGTGCGAGGAGAACTAC | GAAGCACCAGGATCCCATTC |
SMS1 | GCATATTACATCACCACGAGACTCTT | GGAGGTTCATCTGGGAAGCTT |
SMS2 | TTCAGCGGTCACACGGTTAC | ACGAGGCGAATATTCTTTGATGA |
