Figure 9

BHLHE40 facilitates the proliferation, migration and apoptosis of pancreatic cancer cells. (A) BHLHE40 expression in PATU-8988 cells knockdown of BHLHE40. (B,C) Cell Counting Kit-8 (B) and colony formation (C) were used to detect the cell viability of PATU-8988 (NC, sh1-BHLHE40 and sh2-BHLHE40) at the indicated timepoint. (D) Wound healing assay of PATU-8988 cells (shNC, sh1-BHLHE40, sh2-BHLHE40). Photos were taken at 0 and 24 h. (E,F) Flow cytometry was used to analyze the apoptosis rate in PATU-8988 cells transfected with sh1-BHLHE40, sh2-BHLHE40 and negative control. (G) Representative images of xenograft tumors derived from BHLHE40 knocked down and negative control PATU-8988 cells that were subcutaneously injected into BALB/c athymic nude mice (n = 5). (H,I) Tumor volumes and tumor weights of xenograft tumors derived from the BHLHE40 knocked down and negative control in PATU-8988 are shown. Tumor volumes were calculated as volume = length × (width)²/2. The data are represented as mean ± SD.