Table 1 Relative primers used for PCR, real-time PCR, stem-loop sequences (All primer sequences are from human).
Name | Sequences |
|---|---|
U6 | F: CTCGCTTCGGCAGCACA R: AACGCTTCACGAATTTGCGT |
H19 | F: GGAGTGAATGAGCTCTCAGG R: CTAAGGTGTTCAGGAAGGCC |
GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) | F: ACAACTTTGGTATCGTGGAAGG R: GCCATCACGCCACAGTTTC |
β-Tubulin | F: CGGGCAGTGTTTGTAGACTTGG R: CTCCTTGCCAATGGTGTAGTGC |
PGK1 (Phosphoglycerate kinase 1) | F: TGGACGTTAAAGGGAAGCGG R: GCTCATAAGGACTACCGACTTGG |
PDK1 (Pyruvate dehydrogenase lipoamide kinase isozyme 1) | F: CTGTGATACGGATCAGAAACCG R: TCCACCAAACAATAAAGAGTGCT |
PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3) | F: TTGGCGTCCCCACAAAAGT R: AGTTGTAGGAGCTGTACTGCTT |
SLC2A1 (Solute carrier family 2, facilitated glucose transporter member 1) | F: GGCCAAGAGTGTGCTAAAGAA R: ACAGCGTTGATGCCAGACAG |
miRNA-19a-3p | RT: GTCGTATCCAGTGCAGGGTCCGAG GTATTCGCACTGGATACGACACGTGT F: AGTCAAAACGTATCTAA R: GTGCAGGGTCCGAGGT |
miRNA-193a-3p | RT: GTCGTATCCAGTGCAGGGTCCGAG GTATTCGCACTGGATACGACGGTCAA F: TGACCCTGAAACATCC R: GTGCAGGGTCCGAGGT |
miRNA-19b-3p | RT: GTCGTATCCAGTGCAGGGTCCGAG GTATTCGCACTGGATACGACACGTGT F: AGTCAAAACGTACCTAA R: GTGCAGGGTCCGAGGT |
