Figure 1 | Scientific Reports

Figure 1

From: Burkholderia pseudomallei biofilm resists Acanthamoeba sp. grazing and produces 8-O-4′-diferulic acid, a superoxide scavenging metabolite after passage through the amoeba

Figure 1

Schematic flow chart of the co-cultivation of two different B. pseudomallei biofilm phenotypes and Acanthamoeba sp. Adhesion and intracellular-survival assays at MOI 100 using non-encapsulated biofilm cells were performed. Burkholderia pseudomallei were passaged through Acanthamoeba sp. up to three times and were then collected for metabolomic analysis using ultra-high-performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry in parallel with observations of colony morphology on Ashdown’s agar. In addition, preformed 24-h and 48-h B. pseudomallei biofilm was cocultured with Acanthamoeba sp. to monitor the biofilm structure and biofilm biomass using confocal laser scanning microscopy. Amoeba cells were counted using a hemocytometer.

Back to article page