Table 1 Effect of supplementation of Vit. E and C to tris-based extender on chilled semen quality of Kail rams.

From: Effects of vitamins C and E in tris citric acid glucose extender on chilled semen quality of Kail ram during different storage times

Parameters

Groups

Time (h)

P-value

0

24

48

72

G

T

G*T

Motility (%)

A

83 ± 5.4A

76 ± 2.7AB

72 ± 4.8 ± AB

53 ± 3.5B

0.007

0.000

0.000

B

86 ± 1.7A

76 ± 1.9AB

65 ± 5.2AB

51 ± 2.1C

C

78 ± 2.5A

74 ± 7.0AB

61 ± 0.9AB

57 ± 0.7C

D

82 ± 4.2A

70 ± 6.9A

78 ± 5.9A

66 ± 4.6A

PM (%)

A

42 ± 6.4A

30 ± 3.4AB

26 ± 2.3AB

15 ± 3.9C

0.694

0.000

0.183

B

47 ± 3.9A

26 ± 3.4BC

25 ± 3.0CB

12.4 ± 2.8D

C

40 ± 2.3A

31 ± 3.8AB

25 ± 7.7AB

17 ± 6.3C

D

34 ± 3.8A

25 ± 2.1AB

26 ± 5.9AB

21 ± 3.9B

RP (%)

A

5.2 ± 0.7A

3.1 ± 1.0AB

2.4 ± 0.7AB

0.7 ± 0.1C

0.378

0.003

0.132

B

4.6 ± 1.0A

2.4 ± 0.9AB

1.9 ± 0.8AB

0.0 ± 0.6B

C

3.9 ± 0.4A

3.4 ± 0.8A

3.3 ± 0.4A

2.3 ± 0.3A

D

3.1 ± 0.6A

2.8 ± 0.6A

4.4 ± 2.3A

2.6 ± 2.1A

MP (%)

A

37.4 ± 5.9A

27.7 ± 2.4AB

24.1 ± 2.0AB

14.8 ± 3.8B

0.466

0.000

0.361

B

42 ± 3.1A

22.0 ± 2.8BC

24 ± 3.3CB

2.4 ± 2.6D

C

36.1 ± 2.3A

35.7 ± 3.8AB

27.7 ± 12.2AB

13.8 ± 10.8B

D

31.3 ± 3.9A

24.9 ± 7.5A

19.6 ± 5.8A

18.5 ± 4.8A

  1. Data were expressed as mean ± SEM.
  2. Group A = control; Group B = vit. C 1 mg/ml; Group C = vit. E 1 mg/ml; Group D = vit. C + E; G group, T storage time, G*T group storage time interaction, PM progressive motile sperm, RP rapid progressive sperm, MP medium progressive sperm.
  3. Different superscripts ABC within a row indicates significant difference (P < 0.05) within a specific group at different time intervals.