Figure 5
The degeneration of SGNs in control and CL-treated mice after kanamycin injection. (a) ATF3 (red) and βIII-tubulin (green) expressions in the SGNs 3 days after kanamycin injection. Scale bar = 50 μm. (b) The number of ATF3-positive cells/the number of βIII-tubulin-positive cells in apex, middle, and basal turns, and the average of all turns 3 days after kanamycin injection. The ratio is significantly lower in CL-treated mice. N = 4 each, *p < 0.05 by two-way ANOVA followed by Bonferroni post hoc test. (c) Changes of ABRs in control and CL-treated mice 28 days after kanamycin injection. The ABR thresholds in control mice (orange circles) were elevated using 10–15 dB SPL compared with those in CL-treated mice (blue circles) across all frequencies. N = 4 each, *p < 0.05 by two-way ANOVA.
