Figure 3
Effects of a plant-specific extended loop on the kinetic stability of FADH−. (a) Structural comparison of At64 (PDB: 3FY432, in green) and CraCRY in complex with 8-HDF (PDB: 6FN227, in magenta) shows the presence of the extended loop around the secondary pocket in At64. (b) Spectral changes associated with oxidation of the photoreduced At64-ΔExLoop sample were recorded every 10 min. An inset shows the difference spectra in which each spectrum was subtracted by the spectrum at 0 min recorded at wavelengths from 400 to 700 nm.
