Table 4 Feather DNA extraction and PCR results from feathers kept at different temperature conditions for 2, 4 and 6 months.
From: A non-invasive feather-based methodology for the detection of blood parasites (Haemosporida)
Storage time | 0 monthsa | 2 months | 4 months | 6 months | ||||
|---|---|---|---|---|---|---|---|---|
DNA (ng/µl) | PCR result | DNA (ng/µl) | PCR result | DNA (ng/µl) | PCR result | DNA (ng/µl) | PCR result | |
Freezer | 230 .2 315.8 (273) | (+) (+) | 324.6 203.8 (264.2) | (+) (+) | 336.4 253.1 (294.8) | (+) (+) | 147.2 337.7 (242.5) | (+) (+) |
Room temperature | 66.8 28.4 (47.6) | (+) (+) | 53.9 64.9 (59.4) | (+) (+) | 52.7 39.0 (45.9) | (+) (+) | ||
Outdoors | 16.4 29.1 (22.8) | (−) (−) | ||||||