Table 4 Feather DNA extraction and PCR results from feathers kept at different temperature conditions for 2, 4 and 6 months.

From: A non-invasive feather-based methodology for the detection of blood parasites (Haemosporida)

Storage time

0 monthsa

2 months

4 months

6 months

DNA (ng/µl)

PCR result

DNA (ng/µl)

PCR result

DNA (ng/µl)

PCR result

DNA (ng/µl)

PCR result

Freezer

230 .2

315.8

(273)

(+)

(+)

324.6

203.8

(264.2)

(+)

(+)

336.4

253.1

(294.8)

(+)

(+)

147.2

337.7

(242.5)

(+)

(+)

Room temperature

  

66.8

28.4

(47.6)

(+)

(+)

53.9

64.9

(59.4)

(+)

(+)

52.7

39.0

(45.9)

(+)

(+)

Outdoors

  

16.4

29.1

(22.8)

(−)

(−)

    
  1. Two replicates were used per treatment and DNA concentration averages are shown in parentheses.
  2. aDNA extractions used as a positive control.