Figure 5
The effects of the selinexor (Sel) + idelalisib (Ide) combination are mediated by IkBα and FOXO3A. Primary CLL cells were exposed to selinexor and idelalisib at specified concentrations. IkBα nuclear amount (a,c), NF-kB activity (b,d) and cell viability (f,g) were evaluated after 6 and 12 h. FOXO3A nuclear amount (e) was evaluated after 6 h. In (a), (c) and (e) a representative blot (with relative Unique Patient Number, UPN13 and UNP42), together with the corresponding cumulative band intensity data of 7 independent experiments, respectively, is shown. Box and whiskers plots show median values, 25–75% percentiles, and minimum and maximum values for each group. Repositioned gel lanes are indicated by vertical lines. Asterisks indicate the combinations which determined a significant effect compared to both single agents, at the corresponding concentration. ****p < 0.0001, ***p < 0.001, **p < 0.01 and *p < 0.05.
