Figure 2
From: Subcellular distribution of the rAAV genome depends on genome structure
The optimal condition of transfection for proximity labeling with pAPEX2 and psgITR. (a) Quantification of fluorescence after transfection of pAPEX2 and psgITR, transduction of rAAV (top), the corresponding images of fluorescence microscopy of the cells (bottom), (b) cytotoxicity of transfection agents prepared at two plasmids and rAAV. (c) proximity labeling using APEX2 and DAB with different amounts of psgITR. Dark reaction products indicate DAB polymerization by APEX2 (arrow). Data represent means ± SD. Statistical significance was analyzed by one-way analysis of variance, followed by Turkey’s test [n = 3, * p < 0.05, ** p < 0.01, and *** p < 0.001], the scale bar represents 100 µm.
