Figure 4
From: Subcellular distribution of the rAAV genome depends on genome structure
Investigation of ssAAV2 and scAAV2 genome distribution in HEK293 cells. (a) and (b) Transmission electron microscopic (TEM) images of DAB stained ssAAV2 (a) and scAAV2 (b) genome in HEK293 cells. HEK293 cells were pre-treated with the complex consisting of pAPEX2, psgITR, and Mirus for 42 h and then further incubated in virus-containing media for 6 h. After post-incubation, the cells were fixed, stained, and examined by TEM to visualize DAB stained viral genome. The magnified images on the right side come from the squares in the corresponding images on the left side. The letters PS and DS indicate positive staining and DAB staining respectively. N and C designate the nucleus and cytoplasm, respectively.
