Figure 2
Germline identification using DDX4 antibody. (a) Western blot analysis of germ cell marker, DEAD-box helicase 4 (DDX4) compared to the eukaryotic-cell control β-actin detected in total protein extracted from juvenile sturgeon liver and gonad tissues (L = liver, O = ovary, and T = testis) from female (left) and male (right) specimens. (b) shows the comparison of DDX4 intensities normalized to β-actin intensity across the samples. A cropped blot is shown. A full-length blot is presented in Supplementary Fig. S1 online. (c) After dissociation, cells were fixed and stained with a primary antibody against DDX4 (secondary antibody against rabbit IgG) and counterstained with DAPI, a cell nuclear stain. (d) The resultant cell populations of DDX4-positive cells (DDX4+) were identified from DDX4-negative (DDX4-) cells, and counted under fluorescence microscopy (e, top). Under differential interference contrast (DIC), cell diameters were measured (e, bottom; µM, ± SEM). Under DIC, DDX4+ cells were statistically larger than DDX4- cells (t-test, p < 0.0001).
