Figure 5

Cell recovery outcomes from tissue cryopreservation and use in transplantations. Outcomes of (a) viable cell yield from fresh and post-thawed ovarian sections when cryopreserved with media supplemented with ethylene glycol (EG) or dimethyl sulfoxide (DMSO). (b) The percent of cells labeled with DDX4 (DDX4+) recovered from fresh and cryopreserved ovarian tissue using cryomedia with EG or DMSO. (c) The percent of larvae recipients at three months after transplantations with fluorescently tagged cells incorporated along the gonadal ridge. Letters above each bar represent the statistical similar groups determined by ANOVA, p < 0.05).