Figure 3
From: A memory-improving dipeptide, Tyr-Pro, can reach the mouse brain after oral administration
Absorption of Tyr-Pro into the plasma after oral administration to ICR mice. (a, b) SRM chromatograms of LC–MS/MS analysis of APDS-Tyr-[13C5,15N]Pro into the blood after single oral administration to mice. Representative SRM chromatograms of APDS-Tyr-[13C5,15N]Pro (405.1800 > 285.1480 m/z) in the plasma after single oral administration of 10 and 100 mg/kg for different time points (0–120 min) were shown. The corresponding chromatograms of internal standard (IS) of [13C9,15N]Tyr-Pro (409.1920 > 289.1800 m/z) were inserted as a small window for each chromatogram. The peak of the target molecule is indicated by an arrow. N.D. indicates not detected. The plasma sample was subjected to an APDS derivatization, followed by LC–MS/MS-SRM analysis as described in the Materials and method section. (c) Time course of plasma concentrations of Tyr-[13C5,15N]Pro after single oral administration at doses of 10 (solid line) and 100 mg/kg (dotted line) in mice. The values of each time point at different doses were calculated using the present plasma calibration curve. Each value is expressed as mean ± SEM (n = 3–5).
