Figure 1

Phylogenetic tree of selected AA9 LPMOs. A multiple-sequence alignment of the catalytic domains of 47 LPMOs, including ScLPMO9A and NcLPMO9C, was performed using T-Coffee Expresso and used to create the phylogenetic tree. The colors in the tree represent LPMO regioselectivity on cellulose (blue: C4-oxidizing; red: C1-oxidizing; green: C1/C4-oxidizing; no color: regioselectivity unknown). All four AA9s grouped in the clade containing ScLPMO9A are active on soluble cello-oligosaccharides.