Figure 10
Binding interactions between ScLPMO9A and cellopentaose (Glc5), cellohexaose (Glc6), and xyloglucan tetradecamer (XG14). Binding interactions between the substrates and the LPMO were measured by the number of backbone 1H-15N signals showing significant chemical shift perturbations (CSP ≥ 0.025 ppm), and perturbations corresponding to direct interactions (CSP ≥ 0.063 ppm) when titrated with the substrates. The dissociation constant (Kd) of the interactions was calculated by plotting the combined CSP (Δδcomb in ppm) against the substrate concentration (mM). Schematic presentations of Glc5, Glc6, and XG14 are placed above the plots. β-1,4-linked glucose monomers are shown as blue circles, α-1,6-linked xylosyl substitutions are shown as orange stars, and β-1,2-linked galactosyl substitutions are shown as yellow circles. Note that XG14 is a mixture of oligomers and that the number and positions of xylosyl and galactosyl substitutions are unknown.
