Figure 4
From: MCPIP1 functions as a safeguard of early embryonic development
Overexpression of catalytically active Mcpip1 impairs early development in zebrafish. (a) Schematic diagram of control and Mcpip1 overexpression constructs. The sequences were cloned into the pCS2 expression vector. (b) Real-time PCR analysis of zc3h12a expression levels in uninjected and microinjected control, Mcpip1 WT or Mcpip1 DN mRNA zebrafish embryos at 4 hpf and 6 hpf. n = 3. (c) Representative fluorescence (mTurquoise) images of embryos at 6 hpf. (d) Representative bright field images of embryos at 6 hpf. (e) Percentages of live and dead embryos at 6 hpf. (f) Representative bright field images of embryos at 24 hpf. (g) Graph indicating the percentages of live and dead embryos at 24 hpf. n = 3. Rps11 was used as a reference gene. Data represent the mean ± SD, *p < 0.05, **p < 0.01, ****p < 0.0001 by an unpaired t-test (b) or one-way ANOVA (d,f). Scale bar = approx. 250 µm.
