Figure 1
From: Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells
Impaired insulin secretion of INS-1D cells exposed to adipocyte-derived conditioned medium (D8CM). (A) Insulin secretion from INS-1D cells exposed to 5%, 10% and 20% day eight conditioned medium (D8CM) for 24 h was measured after treating with 3 mM glucose (G3), 25 mM glucose (G25) or 30 mM KCl (K30). (B) Insulin secretion from INS-1D cells exposed to 5% D8CM for 3, 6, 12, 24, 48 h were measured after treatment with G3 or G25 or K30. Insulin secretion is shown as percent of insulin content (secreted insulin/(intracellular insulin content + secreted insulin)). *p < 0.05 vs negative control. Values are shown as the mean ± s.e.m (n = 4). [Ca2 +]i levels of INS-1D cells exposed to 5% D8CM for 24 h were measured after treatment with G25 (C) or K30 (D).
