Figure 5
From: Use of pediatric thymus to humanize mice for HIV-1 mucosal transmission
Human CD14+ cells detected in the peripheral blood of mice. The percentage of hCD14+ cells in the peripheral hCD45+ cell population are shown over time for (A) Leg PedThy, (B) Renal PedThy, (C) HSC, and (D) BLT mice described in Table 1. Each symbol denotes a separate animal. Once reconstitution was reached, animals were challenged with HIV-1 and flow cytometry was no longer performed. (E) Immunofluorescence staining of CD68+ cells (white) and nuclear DAPI stain (blue) in representative sections of intestine from a mouse from each group are shown. The average percentages of CD68+ cells per field of total cells are shown from spleen and intestine sections of mice from each group (n = 2, 5 fields of view/tissue). * denotes p values ≤ 0.05; p values of 0.06–0.09 are shown.
