Table 1 Treatments used in settlement assays with crown-of-thorns starfish larvae (n = 12 independent and randomised wells for each treatment).
From: Induction of larval settlement in crown-of-thorns starfish is not mediated by conspecific cues
Treatment | Description |
|---|---|
Experiment 1 | |
1.1 1-m (alg) | 1-month old herbivorous juvenile (coralline algae spp.) |
1.2 1-y (alg) | 1-year old herbivorous juvenile (coralline algae spp.) |
1.3 1-y (cor) | 1 year-old corallivorous juvenile (Acropora spp.) |
1.4 csw 1-m (alg) | fsw conditioned with 1-month old herbivorous juveniles (1.1) |
1.5 csw 1-y (alg) | fsw conditioned with 1-year old herbivorous juveniles (1.2) |
1.6 csw 1-y (cor) | fsw conditioned with 1-year old corallivorous juveniles (1.3) |
1.7 L. pro | 0.5 × 0.5 cm live coralline algae chip (Lithothamnion cf. proliferum) (positive control) |
1.8 L. pro + csw | Herbivorous 1-y-o juvenile conditioned fsw (1.5) added to coralline algae chip (1.7) |
1.9 fsw | Filtered seawater (negative control) |
Experiment 2 | |
2.1 tube foot | Adult tube foot obtained from single 20 cm Ø male |
2.2 spine | Adult spine piece (1.5 cm length) obtained from single 20 cm Ø male |
2.3 csw | Conditioned seawater from 1200 L flow-through tank containing 12 males (15–35 cm Ø) |
2.4 L. pro | 0.5 × 0.5 cm live coralline algae chip (Lithothamnion cf. proliferum) (positive control) |
2.5 fsw | Filtered seawater (negative control) |
