Figure 1 | Scientific Reports

Figure 1

From: The role and impact of alternative polyadenylation and miRNA regulation on the expression of the multidrug resistance-associated protein 1 (MRP-1/ABCC1) in epithelial ovarian cancer

Figure 1

Determination of ABCC1 expression in ovarian cancer. (a) A schematic showing the approximate location of different sets of primers designed to detect ABCC1. (b) An Ethidium bromide stained gel showing the PCR product of different primer combinations for ABCC1. The PCRs were done in biological duplicates (sample 1 and 2) for different primer sets. (c) Quantitative real-time PCR (qRT-PCR) results on RNA from human fallopian tube fimbria secretory epithelial cells (hFTSECs) and two epithelial ovarian cancer cell lines Caov-3 and Ovcar-3 using primers located in the open reading frame (ORF) and the long 3′UTR. The expression levels were normalized to GAPDH and are relative to the levels of the expression of the full length 3’UTR in hFTSECs using the 2–∆∆Ct method. Shown is the average (n = 3,  ± s.d. with *p < 0.001). (d) Western blot of cell lysates from hFTSECs and the Caov-3 and Ovcar-3 cell lines probed for ABCC1 protein and GAPDH. Densitometric readings for ABCC1 each lane was normalized to those of GAPDH using Image J and the values are shown below the Western Blot.

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