Table 1 Laboratory-colonized Anopheles darlingi with Brazilian Plasmodium vivax infected blood.

From: Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi

 

Isolates

Gametocytes/µL

Blood feeding rate (%)

CI 95% of blood feeding rate

p value

Prevalence (%)

CI 95% of prevalence

p value

Intensity

p value

Oocyst

p value

Sporozoite

Whole blood (WB)

n = 10

105 (0–990)

86.8 (872/1004)

84.6–88.8

0.3154

75.0 (237/316)

69.9–79.4

< 0.0001

10 (1–667)

< 0.0001

3040 (80–29,200)

0.0017

Serum replacement (RS)

85.3 (853/1000)

82.9–87.3

92.4 (267/289)

88.6–94.9

18 (1–1059)

3840 (80–40,900)

Control (WB)

n = 15

180 (30–1500)

84.6 (1191/1407)

82.6–86.4

83.4 (262/314)

78.9–87.1

67 (1–706)

9440 (80–79,040)

PABA

83.3 (1169/1403)

81.2–85.1

0.3376

85.5 (271/317)

81.1–88.9

0.4773

60 (1–604)

 > 0.9999

4880 (80–71,360)

< 0.0001

PABA/PSG

82.7 (1164/1406)

80.7–84.6

0.1815

85.2 (265/311)

80.8–88.7

0.5430

67 (1–645)

 > 0.9999

4960 (80–42,880)

< 0.0001

PABA/SR

78.1 (1097/1404)

75.9–80.2

< 0.0001

95.0 (287/302)

91.9–97

< 0.0001

97 (1–759)

0.0004

8400 (160–68,960)

0.3217

PABA/PSG/SR

75.6 (1063/1405)

73.3–77.8

< 0.0001

92.1 (281/305)

88.5–94.7

0.0010

112 (1–846)

< 0.0001

8333 (133–74,320)

0.0802

PSG/SR

85.2 (1193/1400)

83.2–86.9

0.6751

91.7 (286/312)

88.0–94.2

0.0018

112 (1–1275)

< 0.0001

12,800. (1280–143,360)

< 0.0001

  1. Gametocytes/µL, oocysts per mosquito and sporozoites per mosquito are expressed as median (max–min).
  2. PABA = mosquitoes treated with 0.05% para-aminobenzoic acid; PSG = mosquitoes treated with 10 U/mL–μg/mL Pen-Strep and 15 μg/mL of gentamicin.