Table 2 Characteristics of included studies.

From: Early biomarkers for prediction of severe manifestations of dengue fever: a systematic review and a meta-analysis

Study

Method of confirmation of dengue diagnosis

Country

Recruitment time

Clinical classification system

Number of patients

Included in meta-analysis? (yes/no)

Biswas, 201513

RT-PCR, viral isolation, IgM ELISA

Nicaragua

2005–2013

Both 1997 and 2009

789

Yes

Chaiyaratana, 200814

IgM, IgG ELISA

Thailand

2002–2005

1997

177

No

Conroy, 201515

IgM ELISA, viral isolation

Colombia

Not mentioned

1997

111

Yes

Cui, 201616

RT-PCR, NS1 detection

Singapore

Not mentioned

1997

116

Yes

Han, 201917

IgM, NS1 Elisa,

China

2016

1997

36

No

Hapugaswatta, 202118

NS1 rapid test

Sri Lanka

2015–17

2009

127

No

Houghton, 201019

RT-PCR

Colombia

2005–2006

1997

38

No

Koraka, 200420

IgM, IgG ELISA

Indonesia

1995–96

1997

32

No

Kularatnam, 201921

IgM ELISA

Sri Lanka

2013–14

1997

130

No

Kumar, 201222

RT-PCR

Singapore

2005–06

1997

58

Yes

Lam, 202023

RT-PCR, NS1 antigen, IgM assays

Vietnam

2013–15

2009

75

Yes

Laur, 199824

RT-PCR, IgM assay,

Tahiti

1996–97

1997

52

No

Liao, 201525

Antibody tests, virus isolation in C6/36 mosquito cells

China

2009

2009

51

No

Lin, 201926

NS1 Ag STRIP, RT-PCR, and serology

Taiwan

2014—2016

2009

108

Yes

Mapalagamage, 201827

IgM ELISA, rapid immunochromatography test (NS1)

Sri Lanka

2014–16

2009

138

No

Mapalagamage, 202028

IgM ELISA/rapid chromatographic detection NS1

Sri Lanka

2015–16

1997

80

Yes

Mariappan, 202129

NS1 ELISA, IgG, IgM ELISA

India

Not mentioned

2009

47

Yes

Nhi, 201630

serologic assays, virus isolation, RT-PCR

Vietnam

2011–12

2009

63

No

Pang, 201631

RT-PCR, IgM, IgG ELISA

Singapore

2005–08/2009–12

2009

158

No

Rathore, 202032

NS1 ELISA, RT-PCR

Sri Lanka

Not mentioned

1997

84

No

Saniathi, 202133

Not mentioned

Indonesia

2015–16

1997

80

Yes

Sigera, 201934

RT-PCR

Sri Lanka

2017–20

2009

32

Yes

Silva, 202135

NS1 antigen test,RT-qPCR

Sri Lanka

2018–19

2009

120

No

Suwarto, 201736

RT-PCR, IgG, IgM ELISA

Indonesia

2013–15

1997

103

Yes

van de Weg, 201437

RT-PCR, ELISA

Brazil

2011–12 Aruba/2010Brazil

2009

44

No

Villamor, 201738

NS1, RT-PCRIgM ELISA

Colombia

2003–09/2009–10

1997

345

Yes

Villamor, 201839

NS1, IgM ELISA, RT-PCR

Colombia

2003–09/2009–10

1997

344

No

Villar-Centeno, 200840

viral isolation, IgM assays

Colombia

Not mentioned

1997

201

Yes

Vuong, 202141

NS1 IgM ELISA, RT-PCR, viral isolation

Vietnam, El Salvador, Cambodia, Malaysia

2011–15

2009

281

Yes

Vuong, 202042

NS1 ELISA, RT-PCR

Vietnam, El Salvador, Cambodia, Malaysia

2011–15

2009

1117

Yes

Yacoub, 201743

NS1 ELISA, RT-PCR

Vietnam

2013–15

2009

293

No

Yacoub, 201644

RT-PCR, NS1 antigen or DENV IgM, IgG assays

Vietnam

2013–14

2009

132

Yes

Yamanaka, 201345

RT-PCR-NS1, IgM, IgG ELISA

Indonesia

2008–10

1997

130

Yes

Zain, 201746

IgM IgG ELISA for serotyping only

Indonesia

Not mentioned

2009

42

No

Chandrashekhar, 201947

IgG, IgM ELISA, NS1

India

2016–2018

2009

77

No

Fernando, 201648

IgG, IgM, NS1 ELISA,

Sri Lanka

2015

2009

30

Yes

Low, 201849

NS1 ELISA, RT-PCR

Malaysia

2016–2017

2009

82

No