Figure 5
From: Hippophae rhamnoides reverses decreased CYP2D6 expression in rats with BCG-induced liver injury
Effects of HRP on the metabolic activity of CYP2D6 in rats with BCG-induced immune-mediated liver injury. (A) Blood samples were collected 0.25, 0.5, 1, 2, 4, 6, 8, and 12 h after DEM administration. Plasma dextromethorphan was measured using HPLC as described. Data are expressed as the mean ± standard deviation (SD), n = 6. *P < 0.01 compared with the control group. # P < 0.01, compared with the BCG group. Significance was determined by one-way analysis of variance (ANOVA), followed by Tukey’s test. (B) Parameters (AUC, CL, Cmax, MRT, Vd, and T1/2) were calculated from the plasma dextromethorphan concentration curve as described. Data are expressed as the mean ± SD (n = 6 rats). (C) Metabolic rate was expressed by the amount of dextromethorphan metabolized after incubation with microsomes in vitro and was normalized according to the control group. Data are expressed as the mean ± SD (n = 6 rats). AUC Area under the curve; BCG Bacille Calmette-Guerin; CL Clearance rate; Cmax Maximum blood concentration; DEX Dextromethorphan; HPLC High-performance liquid chromatography; HRP Hippophae rhamnoides; MRT Resident time in vivo; T1/2 Half-life; Vd Apparent volume of distribution.
