Figure 6
Analysis of 3D structure of PoxCYP18 and its different mutants. (a) An overview of 3D structure of PoxCYP18 showing secondary structure elements. (b) PoxCYP18 with a focused view of active site residues and secondary structure segments harboring different residues. Yellow colored segment depicts the presence of Cys45 and the active site residues Arg60, Phe65, Met66 and Gln68. Cyan colored segment contains Cys170 and the active site residues viz., Ala106, Phe118, Trp126, Leu127 and His131. (c) 3D structure alignment of PoxCYP18 (purple), PoxCYP18C45S (green), PoxCYP18C170S (cyan) and PoxCYP18C45S/C170S (blue). The overall 3D structure is conserved in all the models, with differences observed in the loop regions, specifically, the one containing Phe118, Trp126, Leu127 and His131 amino acids (circled red). (d) Comparison of root mean fluctuation plots of amino acid residues in PoxCYP18 (black), PoxCYP18C45S (green), PoxCYP18C170S (cyan) and PoxCYP18C45S/C170S (blue).
