Figure 1 | Scientific Reports

Figure 1

From: Mobility gene expression differences among wild-type, Mmp20 null and Mmp20 over-expresser mice plus visualization of 3D mouse ameloblast directional movement

Figure 1

Generation of Amelx-promoter tdTomato (AT) mice. (A) An illustration of amelogenesis stages with the secretory stage highlighted in red to represent Amelx-promoter driven tdTomato expression. (B) Amelx in situ hybridization in an adult mouse incisor. Scale bars: 200 μm (upper); 50 μm (lower). (C) Schematic of the Amelx-tdTomato reporter construct. (D) Adult mouse incisor frozen sections showing the high tdTomato expressing transgene (AT3) and the low expressing transgene (AT4); blue, DAPI stain. Scale bars: 300 μm. (E) Mouse mandible frozen sections at the age of five days showing the AT3 and AT4; blue, DAPI stain. Boxes inside panels are magnified in panels beneath. A vertical arrow indicates Tomes’ processes. Scale bars: 300 μm (upper); 50 μm (lower). (F) Photos of control and AT3 5 day old mouse mandibular area illuminated by an LED light (LED530-3WRF, OptoCode). A horizontal arrow indicates the region where tdTomato is expressed. (G) Mandibular incisors from 7-week-old mice that were calcein labeled by intraperitoneal injections (10 mg/kg; Dojindo) and dissected. The incisors were stained with Hoechst33342 and observed using fluorescence microscopy without fixation. Upper panels were merged with tdTomato (red), calcein (green) and Hoechst33342 (blue). Lower panels are labeled with tdTomato. Arrows indicate regions of tdTomato expression and the regions were magnified in the boxes located to the left top side. Scale bars: 1 mm. (H) Relative gene expression level of amelogenin was analyzed by qPCR to compare between the sorted AT ( +) and AT (−) cells (n = 5 mice per group). Gapdh was used to normalize the results. Am, ameloblast; SI, stratum intermedium; En, enamel. **p < 0.01.

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