Figure 2 | Scientific Reports

Figure 2

From: Mobility gene expression differences among wild-type, Mmp20 null and Mmp20 over-expresser mice plus visualization of 3D mouse ameloblast directional movement

Figure 2

3D imaging of a fixed AT mouse mandible. (A) A mandible from a 7-week-old Mmp20+/+-AT4 mouse was cleared with CUBIC. Upper panels, before (left) and after (right) clearing. Lower panel, phase contrast view of the cleared mandible. Scale bar: 1 mm. (B) Cleared mandibles from 5 day old Mmp20+/+-AT4 and Tg(Amelx-Mmp20)AT4 mice were imaged using a light-sheet fluorescence microscope. Magenta, tdTomato; green, autofluorescence. The molar and incisor were magnified to better observe the ameloblasts. Dash lines along the incisors outline the buccal surface of ameloblasts.

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