Table 1 Cytotoxic effects of the compound BPA on three human cancer cell lines in the MTT assay.

From: Discovery of 1-(5-bromopyrazin-2-yl)-1-[3-(trifluoromethyl)benzyl]urea as a promising anticancer drug via synthesis, characterization, biological screening, and computational studies

Cytotoxicity (IC50 in µM) of BPA against Several Cancer Cellsa

Sr. no.

Cell line typeb

IC50 (µM) valuec

1

MCF-7

29.10 ± 0.18

2

HeLa

32.84 ± 0.02

3

Jurkat

41.07 ± 0.11

4

NIH-3T3d

95.2 ± 0.12

  1. aCytotoxicity of BPA was measured by MTT assay against each cell line.
  2. bThe standard anticancer drugs (positive controls) used for the different cell lines were: Tamoxifen—MCF-7 cells; Avastin—HeLa cells; and Abitrexate—Jurkat cells. On the other hand, DMSO was used as a placebo drug (vehicle control), which showed very negligible cytotoxicity.
  3. cAverage IC50 values (expressed in µM) are indicated in plus or minus “ ± ” standard deviation (SD). Statistically significant values are expressed as *p < 0.05 and **p < 0.01.
  4. dNIH/3T3 is a mouse embryonic fibroblast cell line that was isolated from a mouse NIH/Swiss embryo (i.e., cells that were derived from a Swiss mouse embryonic tissue). Another control with NIH-3T3 cells (with BPA administered) was run in parallel.
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