Figure 3
Dose-dependent myelin wrapping of AAs in response to compounds. (A) 3-point series of concentration (taken from 9-point dose response data) illustrate compounds dose-dependent effect on myelin wrapping. (B–D) Top panel: examples of sigmoidal dose–response curves fitted to the wrapping index data for the 9-point concentration series, for, tasin-1, clemastine and T3. The curves for all the tested compounds are shown in Supplementary Fig. S1; The sigmoidal fit excludes concentrations above which the drug induced cytotoxicity. Reported are average values over all fields of view (3 replicates with combined 27 fields of view). Error bars are standard error of the mean (SEM); Middle panel: examples of MBP staining of myelin wrapping around artificial axons (shown is one field of view at a selected z-plane) corresponding the maximum efficacy dose for tasin-1 (111 nM), clemastine (3 µM) and T3 (111 nM); Bottom panel: processed images from middle panel showing wrapped myelin membrane around artificial axons. Scale bars: main image: 200 μm, insert: 100 μm.
