Figure 2
From: miR-199a and miR-199b facilitate diffuse gastric cancer progression by targeting Frizzled-6
miR-199a and miR-199b suppress GC cell adhesion. (A) Heatmap showing mRNA sequencing results in SNU601-vec, SNU601-miR199a, and SNU601-miR199b cells. Pink, increased expression level; black, decreased expression level (fold-change ≥ 2, P ≤ 0.01). (B) Rank-rank hypergeometric overlap analysis of two profiling datasets: SNU601-vec versus SNU601-miR199a (x-axis; 199a vs. vec) and SNU601-vec versus SNU601-miR199b (y-axis; 199b vs. vec). Hypergeometric P-value is indicated in the color scale. (C) Gene ontology (GO) enrichment analysis of 484 genes downregulated in both SNU601-miR199a and SNU601-miR199b compared with SNU601-vec. Enriched GO terms were analyzed using Metascape (https://metascape.org). Genes associated with the GO terms are shown in the left bar graph for downregulated genes (% in selection, pink) and for the total set of 28,133 genes (% in total, grey). Adjusted P-values are represented in the right bar graph (green). (D) Heatmap showing the qRT-PCR results of cell adhesion-related genes in SNU601-vec, SNU601-miR199a, and SNU601-miR199b cells. Pink, increased expression level; black, decreased expression level. (E) Cell attachment assay with SNU601-vec, SNU601-miR199a, and SNU601-miR199b cells. Cells (3 × 105/well) were seeded on collagen-coated 24-well plates, and non-adherent cells were removed by washing with PBS after 30 min. Attached cells were measured by crystal violet staining. Data are mean + SD (n = 3). P, two-tailed Student’s t-test. (F) Cell detachment assay with SNU601-vec, SNU601-miR199a, and SNU601-miR199b cells. Cells (1 × 105/well) were cultured on 24-well plates for 24 h and then treated with trypsin/EDTA (0.025%/0.265 mM) for 1 or 2 min. Attached cells were measured by crystal violet staining. Data are mean + SD (n = 3). P, two-way ANOVA.
