Figure 1

Electrophoretic separation of pepper protein extracts and their IgE-reactivity with human sera (pooled): (A) Tricine-SDS-PAGE electropherogram of protein isolates (20 µg); (B) IgE-immunoreactive spices protein immunoblot. The underline bands in figure (A) corresponding to IgE-immunoreactive bands in figure (B) were subjected to LC–MS/MS identification. DMP dried mild paprika, DSP dried spicy paprika, DCP dried chili paprika, MW molecular weight marker. Full-length gel and blot are presented in Supplementary Fig. S1.