Table 1 Solanaceae proteins identified by LC–MS/MS analysis and MASCOT software in the IgE-reactive bands.

2

XP_009603582

11S globulin seed storage protein 1-like [Nicotiana tomentosiformis]

278

5

0.25

5

74 (90)—prunin 1 Pru du 6.0101 [Capsicum annuum]/ XP_016570626

56.3

2, 3;

1, 4, 5, 6;

1, 3, 5;

3;

2, 4, 5

XP_009783514;

XP_009624040;

XP_004247523;

XP_006351695;

XP_006351693

11S globulin seed storage protein 2-like [Nicotiana sylvestris, PREDICTED; N. tomentosiformis; Solanum lycopersicum; Solanum tuberosum, PREDICTED]

102–510;

179–254;

122–376;

287;

104–274

3–10;

3–4;

3–8;

6;

2–5

0.25–0.7;

0.16–0.26;

0.26–0.47;

0.36;

0.17–0.37

4–12;

7–8;

8–12;

10;

7–10

81 (91)/ 80 (92)/ 80 (100)/ 81 (100)/ 78 (99)—11S globulin seed storage protein 2 [Capsicum baccatum]/ PHT52858

53.7

2;

1, 3, 5

XP_009624043;

XP_009624041

11S globulin seed storage protein Jug r 4-like [N. tomentosiformis]

377;

94–235

7;

3–8

0.48;

0.17–0.27

14;

5–6

85 (96)—11S globulin seed storage protein Jug r 4-like [C. annuum]/ XP_016565474

52.9

1–6;

1, 3, 4, 5, 6

XP_004247735;

XP_009603583

11S globulin subunit beta [S. tuberosum]; 11S globulin-like [N. tomentosiformis]

109–468; 136–426

2–7;

3–8

0.16–0.34; 0.24–0.42

5–8;

7–12

78 (96)/ 77 (96)—11S globulin seed storage protein Ana o 2.0101 [C. annuum]/ XP_016565958

57.9

1–6

XP_004246943

12S seed storage protein CRA1-like [S. lycopersicum]

130–643

4–32

0.27–0.74

9–15

87 (96)—hypothetical protein BC332_07738 [Capsicum chinense]/ PHU22631

52.9

3

O82013

17.3 kDa class II heat shock protein [Solanum peruvianum]

142

2

0.62

25

89 (100)—17.3 kDa class II heat shock protein [C. annuum]/ XP_016563710

17.7

3

XP_006360819

17.4 kDa class I heat shock protein [S. tuberosum, PREDICTED]

78

4

1.59

23

88 (100)—18.5 kDa class I heat shock protein [C. baccatum]/ PHT28807

18.1

3

XP_004236141

22.7 kDa class IV heat shock protein [S. lycopersicum]

100

2

0.48

13

84 (100)—22.0 kDa class IV heat shock protein [C. chinense]/ PHT97509

22.1

3

NP_001315984

2-Cys peroxiredoxin 1 [S. lycopersicum]

73

3

0.53

13

90 (92)—2-Cys peroxiredoxin BAS1, chloroplastic [C. annuum]/ XP_016543590

29.0

1

P93373

Actin-54 [Nicotiana tabacum]

166

5

0.75

19

98 (100)—actin-7 [C. annuum]/XP_016565383

41.8

1

CAI48071

Anionic peroxidase [C. chinense]

160

4

0.7

18

100—anionic peroxidase [C. chinense]/CAI48071

31.0

2

CAA63710

Annexin [C. annuum]

169

4

0.6

14

100—annexin [C. annuum]/CAA63710

35.9

1, 2

XP_004230031

Aspartyl protease AED3 [S. lycopersicum]

86–95

2

0.19

5

85 (100)—aspartyl protease AED3 [C. annuum]/XP_016557272

47.7

2

AAS20585

Basic beta-1,3-glucanase, partial [C. annuum]

222

3

0.61

22

99 (96) – lichenase [C. annum]/XP_016563240

76 (96)—basic beta-1,3-glucanase [C. annuum]/AAF34761

40.6

39.2

2

AAR90844

Chitinase class I, partial [C. annuum]

76

1

0.37

14

100—basic 30 kDa endochitinase precursor [C. annuum]/ NP_001311510

34.6

1, 2, 4, 5, 6

XP_009630003

Cocosin 1-like [N. tomentosiformis]

186–292

4–6

0.25–0.45

9–10

79 (95)—prunin 1 Pru du 6.0101 [Capsicum annuum]/ XP_016570626

56.3

2

XP_004230208

Desiccation-related protein PCC13-62-like [S. lycopersicum]

125–249

2–3

0.27

8–9

83 (98)—desiccation-related protein PCC13-62 [C. annuum]/ KAF3614133

37.9

1

NP_001234080

Enolase [S. lycopersicum]

117

2

0.19

6

98 (100)—enolase [C. annuum]/ XP_016542903

47.8

2, 3

CAA50750

Fibrillin [C. annuum]

67–332

1–9

0.13–1.59

4–39

100—fibrillin [C. annuum]/ CAA50750

35.3

2

XP_006362723

GDSL esterase/lipase At1g71250 [S. tuberosum, PREDICTED]

157

3

0.38

14

90 (99)—GDSL esterase/lipase At1g71250 [C. annuum]/ XP_016563440

40.8

3

AAN39918

Glutathione S-transferase [C. annuum]

46

1

0.18

7

100—glutathione S-transferase [C. annuum]/ AAN39918

24.9

3

AAD50436

Hypersensitive response assisting protein [C. annuum]

61–88

1–2

0.15–0.32

5–9

100—hypersensitive response assisting protein [C. annuum]/ AAD50436

29.9

2

XP_004247663

Late embryogenesis abundant protein 31-like [S. lycopersicum]

127

2

0.37

13

87 (96)—late embryogenesis abundant protein D-34 [C. chinense]/ PHU21880

26.7

2, 3;

2

XP_009601547; ABI73975

Late embryogenesis abundant protein D-29-like [N. tomentosiformis]; LEA protein 4 [C. annuum]

156–175;

103

3–4;

2

0.57–0.58;

1.72

13–14;

38

72 (85)/ 100—late embryogenesis abundant protein D-29 [C. annuum] XP_016551859

27.6

3;

1, 3, 5;

2, 3, 4, 5, 6

XP_009765013;

XP_004234041;

XP_006356113

Legumin B-like [N. sylvestris, PREDICTED; S. lycopersicum; S. tuberosum, PREDICTED]

124;

143–274;

132–233

4;

4–6;

5–7

0.35;

0.16–0.35;

0.36–0.47

9;

6–8;

6–8

77 (95)/ 82 (100)/ 82 (97)—prunin 1 Pru du 6.0101 [C. annuum]/ XP_016570626

56.3

3

AAT71313

Lipocalin protein [C. annuum]

54

1

0.22

5

100—lipocalin protein [C. annuum]/ AAT71313

21.3

3

ADJ57588

Mitochondrial small heat shock protein [C. annuum]

122

2

0.42

11

100—small heat shock protein, chloroplastic [C. annuum]/ NP_001311883

24.1

2

XP_004230182

NADPH-dependent aldehyde reductase 1, chloroplastic [S. lycopersicum]

155

3

0.4

11

89 (100)—NADPH-dependent aldehyde reductase 1, chloroplastic [Capsicum annuum]/ XP_047250615

37.8

3

XP_004251808

Oleosin 5-like [S. lycopersicum]

124

3

0.99

15

91 (97)—oleosin 21.2 kDa [C. annuum]/ PHT73078

25.4

3

AAF63519;

CAI51309

Pathogenesis-related protein 10 [C. annum; C. chinense]

70;

85

1;

2

0.27;

0.37

8;

9

100—pathogenesis-related protein 10 [C. annum]/AAF63519; 100—pathogenesis-related protein 10 [C. chinense]/ CAI51309

17.3

2

CAB57457

Pectin methylesterase, partial [N. tabacum]

130

3

0.53

14

94 (100)—pectinesterase 2 [C. annuum]/ KAF3647503

29.3

2, 3

ACB30360

PGIP [C. annuum]

53–202

1–5

0.15–0.75

4–20

100—PGIP [C. annuum]/ ACB30360

29.8

1

AAC26785

Phosphoglycerate kinase precursor [S. tuberosum]

118

2

0.18

6

93 (100)—phosphoglycerate kinase, chloroplastic [C. annuum]/ PHT77520

57.1

3

CAA54961

Putative chromoplastic oxydo-reductase [C. annuum]

71

2

0.17

5

100—putative chromoplastic oxydo-reductase [C. annuum]/ CAA54961

56.8

3

CAI48023

Putative pathogenesis related protein [C. chinense]

105

3

1.06

22

100—putative pathogenesis related protein [C. chinense]/ CAI48023

17.2

1, 2

ABQ65860;

XP_004250970

Serine carboxypeptidase III [N. tabacum]; serine carboxypeptidase-like [S. lycopersicum, S. tuberosum, PREDICTED]

86–161;

129

2–3;

2

0.16–0.25;

0.16

4–6;

7

83 (98)/ 85 (97)—serine carboxypeptidase 3 [C. chinense]/ PHU03819

57.1

5

XP_006354928

Serpin-ZX-like [S. tuberosum, PREDICTED]

163

4

0.48

12

91 (100)—serpin-ZX [C. annuum]/ XP_016568712

42.9

3

AAP57477

Small heat shock protein [C. annuum]

54

1

0.27

5

100—small heat shock protein [C. annuum]/ AAP57477

25.9

3

ABY26941

Small heat shock protein class I, partial [C. annuum]

77

2

1.4

26

99 (100)—17.8 kDa class I heat shock protein [C. annuum]/ XP_016577734

17.8

5

NP_001295320

Suberization-associated anionic peroxidase 2 precursor [S. lycopersicum]

232

6

0.72

19

84 (100)—suberization-associated anionic peroxidase 2 [C. annuum]/ PHT90648

38.5

3

AAK97184

Thaumatin-like protein [C. annuum]

85

1

0.17

7

100—osmotin-like protein OSML13 precursor [C. annuum]/ NP_001311827

26.8

3;

3

AAL35363;

AFU48610

Thioredoxin peroxidase [C. annuum; N. tabacum]

95–127

3

0.52–1.04

12–13

100—thioredoxin peroxidase [C. annuum]/ AAL35363; 90 (94)—2-Cys peroxiredoxin BAS1, chloroplastic [C. annuum]/ XP_016543590

17.4; 29.0

1

AAB54016

Transaldolase [S. tuberosum]

211

4

0.42

12

89 (100)—transaldolase [C. chinense]/ PHT54042

48.2

2, 3

XP_009605859

Vicilin Car i 2.0101-like [N. tomentosiformis]

73–114

2–4

0.18–0.28

4–7

68 (97)—vicilin Jug r 2.0101 [C. annuum]/ XP_016567997

47.2

2;

2, 3

XP_009619507; XP_006349017

Xyloglucan endotransglucosylase/hydrolase protein 31-like [N. tomentosiformis; S. tuberosum, PREDICTED]

87;

104–123

3;

2–3

0.45;

0.28–0.45

16;

9–13

91 (93)/ 95 (95)—xyloglucan endotransglucosylase/hydrolase protein 31 [C. annuum]/ XP_016547781

34.2