Table 1 Laboratory evaluation of the Omicron screening assays.

From: Tracking SARS-CoV-2 Omicron lineages using real-time reverse transcriptase PCR assays and prospective comparison with genome sequencing

 

E gene

S:N501Y

S:ins214EPE

S:H69/V70

ORF7b:L11F

M:D3N

Analytical sensitivity (copies/mL)

633

461

132

211

557

2,229

Analytical specificity (%)

1001

100

100

100

100

100

Inter-assay reproducibility (% CV)2

0.30–0.48

0.24–0.84

0.22–0.51

0.77–1.08

0.77–1.08

0.45–0.61

Intra-assay reproducibility (% CV)2

0.05–0.46

0.06–1.28

0.01–0.72

0.26–0.65

0.14–0.87

0.11–0.80

Accuracy (%)

100

100

99.6

100

98.9

99.6

  1. 1The generic E gene marker did amplify with SARS-CoV-1 but this is a known and expected reaction25.
  2. 2The coefficient of variation percentage in CT values was used as a measure of reproducibility.
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