Figure 1
From: Fungicides modify pest insect fitness depending on their genotype and population
Experimental setup for testing how fungicide exposure affects the Colorado potato beetles depending on their parental resistance status and population (not shown on the figure). We genotyped and mated the individuals in the F0 generation, thereafter we mated the beetles according to their insecticide resistance status (i.e., S291G mutation in the acetylcholinesterase-2 gene). Beetles were either heterozygous (RS) or homozygous (RR) for that mutation. Their offspring (F1) were exposed to either control or fungicide treatment and thereafter, we measured their survival, body mass and reproduction (i.e., production of the F2 generation). Beetle images are drawn by Janna Ratavaara.
