Figure 2

The intracellular levels of cysteine, glutathione, H₂S, H₂S₂, H₂S₃, cysteine persulfide, bound sulfane sulfur, and transmitters in KO rats compared to those of the wild-type. a and b. The intracellular concentrations of cysteine (a) and glutathione (b) in the hippocampus measured by HPLC. c to f. Intracellular levels of H₂S (c), H₂S₂ (d), H₂S₃ (e), and cysteine persulfide (f) in the hippocampus were measured using LC–MS/MS. g and h. Intracellular levels of bound (sulphane) sulfur in the hippocampus were measured by gas chromatography. i to n. Relative intracellular concentrations of GABA (i), l-glutamine (j), l-glutamate (k), d-serine (l), l-serine (m), and l-glycine (n) in the hippocampus measured by HPLC. W, wild-type; M, 3MST-KO; T, TRPA1-KO; MT, 3MST/TRPA1-double-KO. The experiments were repeated five times. *p < 0.05, **P < 0.01 significantly different as indicated by the Student's t-test. All data are shown mean ± SEM.