Figure 3

The release of GABA, glutamate and glycine induced by H₂S and H₂S_n from the brain cell suspension and in in vivo microdialysis. a. The release of GABA from the brain cell suspension stimulated by 20 μM each of Na₂S, Na₂S_2, and Na₂S₃ and the changes in the intracellular GABA levels. * p < 0.05, **p < 0.01 with Student's t-test. b to g. The release of GABA (b and e), glutamate (c and f), glycine (d and g) induced by H₂S (b to d) and by H₂S₂ (e to g) at the concentrations indicated in the figure in the wild-type rats (blue line) and TRPA1-KO (orange line). A baseline is defined as 100. (b), n = 5; (c) and (d) n = 7; (e) the wild-type n = 5, TRPA1-KO n = 8; (f) the wild-type n = 6, TRPA1-KO n = 9; (g) the wild-type n = 9, TRPA1-KO n = 8; All data are shown means ± SEM. Each peak value of the response was compared to the corresponding base line response observed at the start of the application of Na₂S or Na₂S₂. *p < 0.05, **p < 0.01, ^#p < 0.1 with Student’s t-test.