Figure 5

Serotonin and GABA regulate a release of H₂S, H₂S₂, H₂S_3, and cysteine persulfide. a to f. Suspended brain cells prepared from the wild-type and 3MST-KO rats were stimulated by 200 μM each of acetylcholine, norepinephrine, GABA, Serotonin (5HT), and glutamate, and released H₂S (a), H₂S₂ (b), H₂S₃(c), cysteine (d), cysteine persulfide (e), and cysteine trisulfide (f) to the medium were measured. g to i. Suspended brain cells prepared from the wild-type rats were stimulated by 200 μM each of acetylcholine and 5HT, and the intracellular levels of H₂S (g), H₂S₂ (h), and H₂S₃ (i) were measured. The experiments were repeated 8–12 times. * p < 0.05, ** p < 0.01 by student t-test. All data are shown mean ± SEM.