Figure 8

Hyperlocomotion induced by MK-801 in 3MST-KO rats. a. Time course of the distance traveled by the wild-type (WT) (n = 12), 3MST-KO (n = 12), and TRPA1-KO (n = 12) rats before and after administration of MK-801(0.2 mg/kg). Two-way ANOVA (genotype × time) showed a significant effect of genotype (F(2, 33) = 16.88, p < 0.01) and time (F(17, 561) = 471.42, p < 0 .01). The interaction between genotype and time was also significant (F(34, 561) = 7.88, p < 0.01). Bonferroni’s post-hoc test revealed that 3MST-KO rats had significantly higher locomotor activity compared to wild-type rats from 10 min after the MK-801 administration onwards. b. Comparison of the total distance traveled by wild-type, 3MST-KO, and TRPA1-KO rats. ANOVA showed that there were significant differences between the groups in the total distance traveled after the injection of MK-801 (F(2,35) = 20.76, p < 0.01) but not before the injection (F(2,35) = 1.13, p = 0.33). Bonferroni's posthoc test showed that the total distance traveled was significantly greater in 3MST-KO rats than in the wild-type and TRPA1-KO rats (p < 0.01). *p < 0.05, **p < 0.01, significantly different as indicated. All data shown are means ± SEM.