Figure 4 | Scientific Reports

Figure 4

From: Withaferin-A attenuates diabetes mellitus induced male reproductive dysfunction mediated by ERα in brain and testes of Swiss albino mice

Figure 4The alt text for this image may have been generated using AI.Figure 4The alt text for this image may have been generated using AI.

Effect of STZ-induced diabetes on testes histopathology by H & E staining; (A) Testis sections of control and WA groups illustrate normal appearance of rounded seminiferous tubules with all testicular cells. In the STZ group, diameter of seminiferous tubules shows significant reductions and the tubules appear distorted. In STZ + WA group, WA treatment shows the restoration among testicular cells in the lumen shown at both 10X and 40X magnifications. Graphs demonstrate. (B) Diameters of seminiferous tubules. (C) Johnsen score. (D) Total sperm count. (E) Sperm motility. (F) Seminiferous epithelium height. (G) Thickness of basement membrane. (H) Immuno-fluorescent localization of ERα in testes. Right upper corner shows expression of ERα in testicular cells at higher magnification. (I) Graph represents the mean from immune-positive cells by integrated optical density (IOD) in arbitrary units (AU). Values are presented as mean ± SD, (n = 6), significance of difference is considered as p ≤ 0.05 (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) with respect to indicated groups. (*, **, and *** indicated the comparison between C and WA, STZ and STZ + WA; #, ##, and ### indicate significant difference from the diabetic (STZ) group at p < 0.05, p < 0.01 and p < 0.001, respectively. [C, Control; STZ, Streptozotocin; WA, Withaferin-A; STZ + WA, Streptozotocin + Withaferin-A].

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