Figure 5
From: High glucose promotes benign prostatic hyperplasia by downregulating PDK4 expression
RG could increase the expression of PDK4 in prostatic epithelial cells and with the ability to influence the level of proliferation, EMT, and apoptosis. (A) The value of OD 450 nm in BPH-1 and RWPE-1 cells after treating with 50 mM Glu and different concentrations of RG for 24 h. (B) The expression of PDK4 in BPH-1 and RWPE-1 cells after treating with different concentrations of RG for 24 h. (C–D) The protein expression of ZO-1, E-cad, and α-SMA calculated by western blot was used to evaluate the influence of RG on EMT. (E–F) the protein expression of BCL2, BAX, and Caspase3 was calculated by western blot to show the influence of RG on the level of apoptosis. *P < 0.05; **P < 0.01; ***P < 0.001. RG = Rosiglitazone, 50 mM Glu = 50 Millimoles per liter of glucose.
