Figure 6
From: High glucose promotes benign prostatic hyperplasia by downregulating PDK4 expression
The glucose and lipid metabolism is affected by high glucose and PDK4 regulation in prostatic epithelial cells. After BPH-1 cells treated with high glucose for 24 h, PDK4-target SiRNA, PDK4-expression plasmid for 72 h, RWPE-1 cells treated with high glucose for 24 h, PDK4-target SiRNA for 72 h, the intracellular level of pyruvic acid (A), lactic acid (B), citric acid (C) was calculated by relative Assay Kit to show the influence of glucose metabolism under corresponding treatment; the mRNA expression of CD36, CPT2, CROT (D–E) was calculated by RT-PCR to show the influence of lipid metabolism under corresponding treatment. *P < 0.05; **P < 0.001; ***P < 0.001.
