Figure 5
Immunofluorescent-stained cryosections of testicular parenchyma from control and busulfan-treated chicks using anti-Nanog antiserum. Testis sections of 6-week old chicks from control and vehicle groups (CON and VE, respectively) showing cytoplasmic expression of NANOG in many germ cells. Marked reduction of germ cells expressing NANOG in testis from chicks treated with busulfan at 10 mg/kg (BU10). Very few cells expressed NANOG in testis sections from chicks treated with busulfan at 20 mg/kg (BU20). No expression of NANOG was detected in chicks treated with busulfan at 40 mg/kg (BU40). Each scale bar indicates 50 μm.
