Figure 6
Relative mRNA expression of genes for pluripotency (c-Myc), primordial germ cells (Nanog), spermatogonial stem cells (GFRα1), apoptosis (Bid) and anti-apoptosis (Bcl-2) in testes from 6-week old chicks treated with fractionated doses of busulfan. (A) Values are expressed as arbitrary units of c-Myc, GFRα1 and Nanog mRNA normalized against the expression levels of GAPDH amplified from the same template, relative to the expression observed in controls. (B) Values are expressed as arbitrary units of Bid and Bcl-2 mRNA normalized against the expression levels of GAPDH amplified from the same template, relative to the expression observed in controls. Asterisks indicate statistically significant differences from control group (CON – P < 0.05). Vehicle injection (VE – DMF + distilled water), busulfan injections at concentrations of 10, 20 and 40 mg/kg (BU10, BU20, and BU40 groups, respectively).
