Table 2 Typical property values used for immunoassay of cTnI.

From: Theoretical analysis of immunochromatographic assay and consideration of its operating parameters for efficient designing of high-sensitivity cardiac troponin I (hs-cTnI) detection

Property

Value

Diameter of tube (Dia) 20

\(800 \mathrm{\mu m}\)

Length of tube \(\left(L\right)\)

\(10 \mathrm{mm}\)

Diffusion coefficient \(\left(D\right)\) 21

\(95\times {10}^{-8} {\mathrm{cm}}^{2}/\mathrm{s}\)

Volumetric FLOW RATE (Q) 20

\(0.25 \mathrm{mL}/\mathrm{min}\)

Inlet concentration (C0)

\(0.42 \mathrm{pM}\) or 1 \(0 \mathrm{ng}/\mathrm{L}\)

Rate constant for different capture antibodies \(\left(k\right)\) 22

 

19C7

\(11.9\times {10}^{5} {\mathrm{M}}^{-1}{\mathrm{s}}^{-1}\)

4C2

\(6.54\times {10}^{5} {\mathrm{M}}^{-1}{\mathrm{s}}^{-1}\)

MF4

\(4.95\times {10}^{5} {\mathrm{M}}^{-1}{\mathrm{s}}^{-1}\)

8E10

\(2.54\times {10}^{5} {\mathrm{M}}^{-1}{\mathrm{s}}^{-1}\)

  1. Suitable variations in these values are considered and explicitly stated wherever applicable.