Figure 3
PC’s Acetylated tubulin correlates with SCR and subfertility. (A) A graph showing the correlation between a bull’s SCR deviation from the mean and the FRAC acetylated tubulin mean ratio in the PC of its high-quality sperm. Each bull is represented by a dot (blue for negative FRAC tests, and red for positive FRAC tests) as well as a 95% confidence interval. Bulls with outlier values are also marked with the name of the outlier biomarker (Tb, tubulin; AT, ace tubulin; PO, POC1B; FA, FAM161A), its location (PC, DC, or Ax), and arrows indicating if the outlier is higher (↑) or lower (↓) than the reference range. The number of arrows indicates the number of SD away from the reference range’s upper or lower bound (rounding up). Bull 2 (#2) which had an abnormal acetylated tubulin distribution is marked (see Fig. 4A). (B) Shown are the four tests, their sensitivity and their odds ratio used to determine fertility status in a bull. In the left column are the criteria each test uses to determine fertility status. In the far-right column are the 95% confidence intervals for the odds ratios (OR) shown in the third column. The test with the highest strength of association to accepted fertility status was based on the 0.31 cutoff generated by linear regression.
