Figure 6

G3BP2 promoted CRC cell stemness. HT29 cells were transfected with si-G3BP2 and SW480 cells were transfected with pcDNA3.1-G3BP2. (A) RT-qPCR and (B) western blot analysis of Oct-4 and Nanog level in transfected cells. (C) Sphere formation efficiency was determined by using the sphere formation assay. *P < 0.05; **P < 0.01; ***P < 0.001.