Figure 2
From: Knockdown of lncRNA MALAT1 attenuates renal interstitial fibrosis through miR-124-3p/ITGB1 axis
(a) The transfection efficiency of knockdown lncRNA MALAT1 was detected by qRT-PCR in HK2 cells. NS = no significance; ***: p < 0.001. (b) The transfection efficiency of knockdown lncRNA Malat1 was detected by qRT-PCR in NRK-49F cells. NS = no significance; ***: p < 0.001. (c) After knockdown of lncRNA MALAT1 expression in TGF-β1 induced HK2 cells, WB showed that the expression changes of fibrosis-related markers (E-ca, N-ca VIM, α-SMA). Each assay was implemented in triplicate (n = 3). (d) After knockdown of lncRNA Malat1 expression in TGF-β1 induced NRK-49F cells, WB showed that the expression changes of fibrosis-related markers (FN, COL1, VIM, α-SMA). Each assay was implemented in triplicate (n = 3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001 (Len-sh-MALAT1group and Len-sh-control + TGF β1 group compare with Len-sh-control); #: p < 0.05; ##: p < 0.01; ###: p < 0.001; ####: p < 0.0001 (Len-sh-MALAT1 group was compared with Len-sh-MALAT1 + TGF β1group); &: p < 0.05; &&: p < 0.01 (Len-sh-control + TGF β1 group was compared with Len-sh-MALAT1 + TGF β1 group).
